Science, Volume 333, Issues 6039-6044John Michels (Journalist) American Association for the Advancement of Science, 2011 |
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Page 229
... ubiquitin binding mutant ( E478G ) ] . p62 LIR ( aa 311 to 444 ) was also included . Interaction was IB : a - GFP Ponceau S assessed using a B - galactosidase assay . ( C ) GST pull - down assay of EGFP- OPTN from stable MCF - 7 cells ...
... ubiquitin binding mutant ( E478G ) ] . p62 LIR ( aa 311 to 444 ) was also included . Interaction was IB : a - GFP Ponceau S assessed using a B - galactosidase assay . ( C ) GST pull - down assay of EGFP- OPTN from stable MCF - 7 cells ...
Page 231
... ubiquitin ( lower panel ) at 4 hpi with cytosolic Salmonella sifA ( blue ) . ( C ) Quantification of colocalization of cytosolic Salmonella sifA with EGFP - OPTN , ubiquitin , and pSer177 OPTN from cells represented in ( B ) , as ...
... ubiquitin ( lower panel ) at 4 hpi with cytosolic Salmonella sifA ( blue ) . ( C ) Quantification of colocalization of cytosolic Salmonella sifA with EGFP - OPTN , ubiquitin , and pSer177 OPTN from cells represented in ( B ) , as ...
Page 232
... ubiquitin - binding - deficient OPTN mutant ( E478G ) or LIR mutant ( F178A ) was not able to rescue Sal- monella suppression to the level of WT OPTN ( Fig . 4C ) . Thus , OPTN requires both Ub and LC3- binding domains to restrict ...
... ubiquitin - binding - deficient OPTN mutant ( E478G ) or LIR mutant ( F178A ) was not able to rescue Sal- monella suppression to the level of WT OPTN ( Fig . 4C ) . Thus , OPTN requires both Ub and LC3- binding domains to restrict ...
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