Science, Volume 333, Issues 6039-6044John Michels (Journalist) American Association for the Advancement of Science, 2011 |
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Page 351
... genome transfer . ( A ) Two pairs of strains illustrate the design and methodology of CAGE , with recoded genomic regions in red . Partially recoded strains are split into conjugation pairs . The donor strain ( D ) contains the ...
... genome transfer . ( A ) Two pairs of strains illustrate the design and methodology of CAGE , with recoded genomic regions in red . Partially recoded strains are split into conjugation pairs . The donor strain ( D ) contains the ...
Page 352
... genome . Before conjugation , we converted the 32 strains that collectively contain all TAG - to - TAA modifications into 16 pairs of strains primed for large - scale genome transfer ( Fig . 1 ) . Within each conjugation pair , a donor ...
... genome . Before conjugation , we converted the 32 strains that collectively contain all TAG - to - TAA modifications into 16 pairs of strains primed for large - scale genome transfer ( Fig . 1 ) . Within each conjugation pair , a donor ...
Page 353
... genomes with up to 80 modifications with mild phenotypic consequences . The scarless introduction of codon changes via MAGE en- abled the first genome - wide allelic replacement frequency map using single - stranded DNA oligos in E ...
... genomes with up to 80 modifications with mild phenotypic consequences . The scarless introduction of codon changes via MAGE en- abled the first genome - wide allelic replacement frequency map using single - stranded DNA oligos in E ...
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