Science, Volume 333, Issues 6039-6044John Michels (Journalist) American Association for the Advancement of Science, 2011 |
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Page 453
... Rab1 . The effector protein SidM recruits Rab1 to the Legionella - containing vacuole ( LCV ) , where it activates Rab1 and then AMPylates it by covalently adding adenosine monophosphate ( AMP ) . L. pneumophila GTPase - activating ...
... Rab1 . The effector protein SidM recruits Rab1 to the Legionella - containing vacuole ( LCV ) , where it activates Rab1 and then AMPylates it by covalently adding adenosine monophosphate ( AMP ) . L. pneumophila GTPase - activating ...
Page 454
... Rab1 generates AMP . AMP was detected by a competitive fluorescence polarization immunoassay . AMP and ADP standards are shown as positive and negative assay controls , respectively . Error bars indicate SD from three independent ...
... Rab1 generates AMP . AMP was detected by a competitive fluorescence polarization immunoassay . AMP and ADP standards are shown as positive and negative assay controls , respectively . Error bars indicate SD from three independent ...
Page 455
... Rab1 inactivation by LepB and removal from sar LCVs . ( A and B ) De - AMPylation enables GAP - stimulated GTP hydrolysis by Rab1 . GTP hydrolysis of Rab1 - AMP ( 1 μM ) loaded with [ y32P ] GTP in the absence or presence of GST - SidD ...
... Rab1 inactivation by LepB and removal from sar LCVs . ( A and B ) De - AMPylation enables GAP - stimulated GTP hydrolysis by Rab1 . GTP hydrolysis of Rab1 - AMP ( 1 μM ) loaded with [ y32P ] GTP in the absence or presence of GST - SidD ...
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