Science, Volume 333, Issues 6039-6044John Michels (Journalist) American Association for the Advancement of Science, 2011 |
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Page 229
... OPTN variants [ pACT2 : full - length OPTN WT , LIR mutant ( F178A ) or ubiquitin binding mutant ( E478G ) ] . p62 LIR ( aa 311 to 444 ) was also included . Interaction was IB : a - GFP Ponceau S assessed using a B - galactosidase assay ...
... OPTN variants [ pACT2 : full - length OPTN WT , LIR mutant ( F178A ) or ubiquitin binding mutant ( E478G ) ] . p62 LIR ( aa 311 to 444 ) was also included . Interaction was IB : a - GFP Ponceau S assessed using a B - galactosidase assay ...
Page 231
... OPTN and mCherry - LC3B 4 hours post infection ( hpi ) with S. Typhimurium ( SL1344 ) . EGFP - OPTN colocalizes with LC3B ( upper panel ) on a fraction of Salmonella . Phospho - Ser177 OPTN localizes to EGFP- expressing Salmonella ...
... OPTN and mCherry - LC3B 4 hours post infection ( hpi ) with S. Typhimurium ( SL1344 ) . EGFP - OPTN colocalizes with LC3B ( upper panel ) on a fraction of Salmonella . Phospho - Ser177 OPTN localizes to EGFP- expressing Salmonella ...
Page 232
... OPTN WT , shR- OPTN F178A , and shR - OPTN E478G . Both LIR mutants and Ub - binding mutants of OPTN failed to rescue OPTN - depleted HeLa cells 8 hpi compared with OPTN siTBK + siOPTN EGFP - OPTN WT . Error bars indicate mean ± SD ...
... OPTN WT , shR- OPTN F178A , and shR - OPTN E478G . Both LIR mutants and Ub - binding mutants of OPTN failed to rescue OPTN - depleted HeLa cells 8 hpi compared with OPTN siTBK + siOPTN EGFP - OPTN WT . Error bars indicate mean ± SD ...
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